Department Name

Jae-Sung Kim Ph.D.

RESEARCH INTERESTS:

Dr. Kim's research focus is to understand the mechanisms underlying ischemia/reperfusion injury to liver and heart. Ischemia/reperfusion injury occurs in many circumstances, including surgery, organ preservation for transplantation, veno-occlusive disease, hemorrhagic shock-resuscitation, and heart failure. Reperfusion of ischemic tissue promotes onset of the mitochondrial permeability transition (MPT), a causative event contributing to both apoptotic and necrotic cell death. Dr. Kim's laboratory is applying laser scanning single and multiphonton confocal microscopy to elucidate cellular and molecular mechanisms leading to the MPT after ischemia/reperfusion. Using isolated hepatocytes and cardiac myocytes, his lab is investigating how ion homeostasis, mitochondrial membrane potential, formation of reactive oxygen and nitrogen species, membrane permeability, redox status and sphingolipid metabolism are involved in the MPT and consequent cell death. Understanding these cellular events may be important in developing new strategies to improve hepatic and cardiac function after lethal ischemia/reperfusion injury.

Support: 

National Institutes of Health (PO1), Molecular Pathogenesis of Apoptosis in Liver Cells 2001-2004

Department of Surgery, UF (Research Career Development Award), Role of Sphingosine and Lysosomes in Ischemia/reperfusion Injury to Hepatocytes 2006 

Publications:

1. Kim J-S, and Southard JH.  Effect of liver preservation on hepatocyte calcium and ATP generation.  Transplant Proc  1997, 29. 3447-3448.

2. Kim J-S, Boudjema K, D'Alessandro A, and Southard JH.  Machine perfusion of the liver: Maintenance of mitochondrial function after 48-hour preservation.  Transplant Proc  1997, 29. 3452-3454.

3. Kim J-S, and Southard JH.  Alteration in cellular calcium and mitochondrial functions in the rat liver during cold preservation.  Transplantation  1998, 65. 369-375.

4. Kim J-S, and Southard JH.  Membrane stabilizing effects of calcium and taxol during the cold storage of isolated rat hepatocytes.  Transplantation  1998, 68. 938-943.

5. Kim J-S, and Southard JH.  Phospholipid metabolism of hypothermically stored rat hepatocytes. Hepatology  1999, 30. 1232-1240.

6. Kim J-S, and Southard JH.  Effect of phospholipase A₂ inhibitors on the release of arachidonic acid and cell viability in cold stored hepatocytes.  Cryobiology  1999, 40. 27-35.

7. Lemasters JJ, Qian T, He L, Kim J-S, Elmore SP et al. Role of mitochondrial inner membrane permeabilization in necrotic cell death, apoptosis and autophagy. Antioxidants & Redox signaling  2002, 4. 769-781.

8. Kim J-S, Qian T, and Lemasters JJ.  Mitochondrial permeability transition in the switch from necrotic to apoptotic cell death in ischemic rat hepatocytes. Gastroenterology 2003, 124. 494-503.(Cover Article and Image)

9. Kim J-S, He L, and Lemasters JJ. Mitochondrial permeability transition: a common pathway to necrosis and apoptosis. Biochem Biophys Res Commu  2003, 304. 463-470.

10. Kim J-S, He L, and Lemasters JJ. Role of the mitochondrial permeability transition in apoptotic and necrotic cell death after ischemia/reperfusion injury to hepatocytes Curr Mol Med  2003 3. 85-94.

11. Kim, J-S, Oshima S, Pediaditakis P, and Lemasters JJ. Nitric oxide protects rat hepatocytes against reperfusion injury mediated by the mitochondrial permeability transition. Hepatology  2004, 39. 1533-1543.

12. Kon, K, Kim J-S, Jaeschke H, and Lemasters JJ. Mitochondrial permeability transition in acetaminophen-induced necrotic and apoptotic cell death to cultured mouse hepatocytes^. Hepatology  2004, 40. 1170-1179 (Cover Article and Image)

13. Kim, J-S, Oshima S, Pediaditakis P, and Lemasters JJ .Nitric oxide: a signaling molecule against the mitochondrial permeability transition-and pH-dependent cell death after reperfusion. Free radical Biology & Medicine  2004, 37. 1943-1950.

14. Kim, J-s, Jin Y, and Lemasters JJ. Reactive oxygen species, but not Ca²⁺ overloading, trigger ph- and mitochondrial permeability transition-dependent death of adult rat myocytes after ischemia/reperfusion. Am J Physiol  2006, In Press.